By Shan-Rong Shi, Clive R. Taylor

ISBN-10: 0470624523

ISBN-13: 9780470624524

ISBN-10: 0470875615

ISBN-13: 9780470875612

The main entire, up to date reference on antigen retrieval and immunohistochemistryAn antigen is a substance that activates the iteration of antibodies and will reason an immune reaction. The antigen retrieval (AR) procedure is in huge use around the globe, and is a severe procedure utilized in scientific analysis of disorder, relatively medical designated melanoma treatment.Antigen Retrieval Immunohistochemistry dependent examine and Diagnostics discusses a number of clinical techniques to the standardization of quantifiable immunohistochemistry (IHC). in response to the advance and alertness of AR by way of the editors, this quantity summarizes contemporary achievements in AR-IHC and analyzes a number of state-of-the-art matters for destiny study projects.Featuring contributions from a world crew of major specialists and learn scientists within the box, this crucial work:Summarizes the foremost difficulties within the 4 fields of antigen retrievalDiscusses the advances of AR innovations and their applicationsProvides useful equipment and protocols in AR-IHC, corresponding to extraction of nucleic acids and proteins for molecular research, cell/tissue pattern training, and standardization and improvement of varied concepts to fulfill the long run wishes of?clinical and study molecular analysisEncourages extra study in AR and IHC, rather how AR tools will be hired for superior try out functionality and the improvement of better reliability and reproducibility of IHCIncludes an appendix of similar laboratory protocolsAntigen Retrieval Immunohistochemistry established study and Diagnostics is meant for medical pathologists, molecular cellphone biologists, uncomplicated learn scientists, technicians, and graduate scholars who adopt tissue/cell morphologic and molecular research and need to take advantage of and expand the ability of immunohistochemistry. it's also pertinent for many biotechnology businesses majoring in improvement of IHC products.Wiley sequence in Biomedical Engineering and Multi-Disciplinary built-in platforms / Kai Chang, sequence Editor

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2003; 36: 1–7. 19. Kim SH. Evaluation of antigen retrieval buffer systems. J. Mol. Histol. 2004; 35: 409–416. 20. Choi JK, Mauger J, McGowan KL. Immunohistochemical detection of Aspergillus species in pediatric tissue samples. Am. J. Clin. Pathol. 2004; 121: 18–25. 21. Gill SK, Ishak M, Rylett RJ. Exposure of nuclear antigens in formalin-fixed, paraffin-embedded necropsy human spinal cord tissue: detection of NeuN. J. Neurosci. Meth. 2005; 148: 26–35. 22. Du J, Shi XY, Zheng J, et al. Antigen retrieval immunohistochemistry under the influence of pH value and time.

Shi SR, Cote RJ, Taylor CR. Antigen retrieval immunohistochemistry: past, present, and future. J. Histochem. Cytochem. 1997; 45: 327–343. 10. Shi S-R, Cote RJ, Chaiwun B, et al. Standardization of immunohistochemistry based on antigen retrieval technique for routine formalin-fixed tissue sections. Appl. Immunohistochem. 1998; 6: 89–96. 11. Shi S-R, Cote RJ, Taylor CR. Standardization and further development of antigen retrieval immunohistochemistry: strategies and future goals. J. Histotechnol.

28. Shi S-R, Cote RJ, Shi Y, et al. Antigen retrieval technique. In Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology, ed. S-R Shi, J Gu, and CR Taylor, pp. 311–333. Natick, MA: Eaton, 2000. 29. Hsi ED. A practical approach for evaluating new antibodies in the clinical immunohistochemistry laboratory. Arch. Pathol. Lab. Med. 2001; 125: 289–294. 30. Namimatsu S, Ghazizadeh M, Sugisaki Y. Reversing the effects of formalin fixation with citraconic anhydride and heat: a universal antigen retrieval method.

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Antigen Retrieval Immunohistochemistry Based Research and Diagnostics by Shan-Rong Shi, Clive R. Taylor

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